Journal article
Ultrasensitive, multiplex Raman frequency shift immunoassay of liver cancer biomarkers in physiological media
B Tang, J Wang, JA Hutchison, L Ma, N Zhang, H Guo, Z Hu, M Li, Y Zhao
ACS Nano | Published : 2016
Abstract
Highly sensitive multiplex biomarker detection is critical for the early diagnosis of liver cancer. Here, a surface-enhanced Raman scattering (SERS) frequency-shift immunoassay is developed for detection of liver cancer biomarkers α-fetoprotein and Glypican-3 down to subpicomolar concentrations in saline solution. A high temperature modification of the Tollen's method affords silver nanoparticle films with excellent SERS response upon which ordered domains of Raman reporters are chemisorbed by microcontact printing. Shifts in the reporters SERS spectrum in response to a bound antibody's biomarker recognition constitutes the frequency shift assay, exhibiting here exceptional sensitivity and s..
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Awarded by Australian Research Council
Funding Acknowledgements
This work was supported in part by the National Basic Research Program of China (no. 2011CB933101, 2015CB932004), National Natural Science Foundation of China (grant no. 21303208). The Project was also sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry (no. Y31Z55). The Start-Up Funding from the Institute of High Energy Physics of the Chinese Academy of Sciences (no. 2011IHEPYJRC504) is also gratefully acknowledged. J.A.H. acknowledges the support of the Australian Research Council DECRA scheme (DE130101300) and the infrastructure support from the Australian Research Council Grant LF 100100117.